Cells were resuspended in 1?ml of 1X PBS and incubated with 10?g/ml PI dye solution (Sigma, St. and incubated at 37C for 10 minutes. A 50 g/ml histone concentration was utilized for activation and cells were incubated immediately for 24 hours. Supernatant was collected for lactate dehydrogenase (LDH) measurements and … Continue reading Cells were resuspended in 1?ml of 1X PBS and incubated with 10?g/ml PI dye solution (Sigma, St